The HydroLAKES database provides a seamless high-resolution map of the world’s lakes and their main characteristics in support of a broad range of global-scale assessments and analyses, with a focus on improving users’ ability to model the important roles that lakes play in the global environment. The current version contains the polygons for 1.5 million individual lakes. HydroLAKES aims to be as comprehensive and consistent as possible at a global scale and contains both freshwater and saline lakes, including the Caspian Sea, as well as human-made reservoirs and regulated lakes. HydroLAKES achieves virtually full completion for lakes above 35 ha in area and close to full completion for lakes between 10 and 35 ha. HydroLAKES is publicly available for download at http://www.hydrosheds.org and is free for scientific, educational, and other uses. The data is licensed under a Creative Commons Attribution 4.0 International License (see section 4). By downloading and using the data the user agrees to the terms and conditions of this license. The copyright © of HydroLAKES is held by the authors, 2016, all rights reserved. Citations and acknowledgements of the HydroLAKES database should be made as follows: Messager, M.L., Lehner, B., Grill, G., Nedeva, I., Schmitt, O. (2016): Estimating the volume and age of water stored in global lakes using a geo-statistical approach. Nature Communications: 13603. doi: 10.1038/ncomms13603. Data is available at www.hydrosheds.org